acrylamide stock solution (Bio-Rad)

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Bio-Rad acrylamide stock solution
Acrylamide Stock Solution, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 280 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/acrylamide+bis+acrylamide+stock+solution/Acrylamide%2FBis+19%3A1+Solution+40%25/10__1016_slash_j__xcrp__2026__103202-266-0-3
Average 99 stars, based on 280 article reviews

acrylamide stock solution - by Bioz Stars, 2026-10

99/100 stars

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Western Blot:

Article Title: Multisite phosphorylation of S6K1 directs a kinase phospho-code that determines substrate selection
Article Snippet: .. Immunoblot analysis Ccell lysates or immunoprecipitates were denatured in Laemmli sample buffer (Bio-Rad) and resolved on Tris-glycine SDS-PAGE (10, 12, or 15% polyacrylamide) prepared using 37.5:1 acrylamide:bis-acrylamide stock solution (National Diagnostics), or on precast Express Plus Tris-MOPS-SDS PAGE (8, 10, 12, 4-12%, 4-20%; GenScript). .. After transfer to polyvinyl difluoride membranes, proteins were probed with target-specific antibody, followed by incubation with horseradish peroxidase-conjugated secondary antibody (GE Healthcare), and detected with ECL prime western blotting detection reagent (GE Healthcare).

SDS Page:

Article Title: EPRS is a critical mTORC1-S6K1 effector that influences adiposity in mice
Article Snippet: Labeled cells were lysed in RIPA buffer (Thermo Fisher) and analyzed by Tris-glycine SDS-PAGE, fixation and autoradiography. .. Cell lysates or immunoprecipitates were denatured in Laemmli sample buffer (Bio-Rad) and resolved on Tris-glycine SDS-PAGE (10, 12, or 15% polyacrylamide) prepared using 37.5:1 acrylamide:bis-acrylamide stock solution (National Diagnostics). .. After transfer to polyvinyl difluoride membrane, the membranes were probed with target-specific antibody, followed by incubation with horseradish peroxidase conjugated secondary antibody and detection with Amersham ECL prime western blotting detection reagent (GE Healthcare).

Article Title: Expanding the Tubulin Code: TTLL11 Polyglutamylase Drives Elongation of Primary Tubulin Chains
Article Snippet: .. α- and β- tubulins were resolved by SDS-PAGE composed of a 10 % acrylamide separation gel (375 mM Tris-HCl, pH 9, 0.1 % SDS (Sigma #L5750)) and 3.5 % stacking gel (125 mM Tris-HCl, pH 6.8, 0.1 % SDS) prepared using the acrylamide/bis-acrylamide stock solution [40 % acrylamide solution (Bio-Rad #161-0140) mixed with 0.54 % w/v bis-acryl amide dry powder (Bio-Rad #161-0210)]. ..

Article Title: Multisite phosphorylation of S6K1 directs a kinase phospho-code that determines substrate selection
Article Snippet: .. Ccell lysates or immunoprecipitates were denatured in Laemmli sample buffer (Bio-Rad) and resolved on Tris-glycine SDS-PAGE (10, 12, or 15% polyacrylamide) prepared using 37.5:1 acrylamide:bis-acrylamide stock solution (National Diagnostics), or on precast Express Plus Tris-MOPS-SDS PAGE (8, 10, 12, 4-12%, 4-20%; GenScript). .. After transfer to polyvinyl difluoride membranes, proteins were probed with target-specific antibody, followed by incubation with horseradish peroxidase-conjugated secondary antibody (GE Healthcare), and detected with ECL prime western blotting detection reagent (GE Healthcare).

Article Title: Mechanistic insights into TTLL11 polyglutamylase–mediated primary tubulin chain elongation
Article Snippet: .. α- and β-tubulins were resolved by SDS-PAGE composed of a 10% acrylamide separation gel [375 mM tris-HCl (pH 9), and 0.1% SDS (Sigma-Aldrich, #L5750)] and 3.5% stacking gel [125 mM tris-HCl (pH 6.8) and 0.1% SDS] prepared using the acrylamide/bis-acrylamide stock solution [40% acrylamide solution (Bio-Rad Laboratories, #161-0140) mixed with 0.54% (w/v) bis-acrylamide dry powder (Bio-Rad Laboratories, #161-0210)]. ..

Polyacrylamide Gel Electrophoresis:

Electrophoresis:

Article Title: JNK does not regulate meiotic progression in Xenopus oocytes: The strange case of pJNK and pERK.
Article Snippet: The supernatants were denatured with Sample Buffer (50 mM Tris HCl, pH 6.8, SDS 2%, 100 mM dithiothreitol, 10% glycerol) at 100 °C for 5 min and subjected to 10% or 15% SDS/PAGE and transferred to Immobilon-P membranes (Millipore). .. Generally, polyacrylamide gels for electrophoresis were prepared from a commercial 40% acrylamide/bis-acrylamide stock solution (37.5:1 acrylamide: bis, Bio-Rad, Cat. no. 161-0148), but a better separation of the 40 and 42 kDa protein bands were obtained with gels prepared from a 30% acrylamide/bisacrylamide solution (100:1 w/w acrylamide: bis, Sigma). .. Uniformity of samples loading was verified by Ponceau (Sigma) staining of the blots.

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